In each area, watersheds are very important because they are the largest physical and topographic units which contain various ecosystems. Soil, the most important component of these ecosystems, is the third source of carbon storage in the world, playing an important role in global balance of carbon and its sequestration. The purpose of this study is to investigate the role of watershed management projects on carbon sequestration, which it could enrich the soil and improve watershed conditions. In order to estimate the soil sequestration in Fars Kelestan watershed, six different land uses including peanuts planting, grape planting, bush planting , milk vetch, natural almonds and seeding were selected. Soil sampling at two depths (0-20 cm), (20-50 cm) were carried out, in which.35 samples stratification collected from each depth. The results revealed that the amount of carbon sequestration varies in different land uses. The maximum amount of carbon sequestration in depth (0-20cm) belongs to bush planting which equals to 64.628 ton/ha. The highest rate of carbon sequestration at depth (20-50cm) belongs to the peanuts planting  which is 57.652 ton/ha. In peanuts planting land use the total carbon sequestration is 119.150 ton/ha. In purpose of carbon sequestration increasing, biological treatments are recommended  with the priority order of the peanuts planting, bush planting, milk vetch, grape planting, seeding were, and natural almonds.